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Table 1 Secretion efficiency and α-amylase activity in recombinant L. plantarum WCFS1 harbouring various constructsa.

From: Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1

Plasmids

Cell fractions

(102 mU ml-1 OD600 -1)

Culture supernatants

(102 mU ml-1 OD600 -1)

Secretion efficiency

(%)c

Ranking number after NucA activity

pLp_3050sAmy

7.2 ± 0.6

0.57 ± 0.04

7

1

pLp_2145sAmy

28.0 ± 3.6

0.92 ± 0.04

3

2

pLp_3189sAmy

8.7 ± 1.0

0.92 ± 0.03

10

3

pLp_3077sAmy

29.9 ± 5.1

0.43 ± 0.04

1

4

pM6sAmyb

53 ± 10

0.40 ± 0.17

0.7

5

pLp_0297sAmy

7.2 ± 2.6

3.4 ± 0.34

32

6

pLp_3117sAmy

6.8 ± 0.8

0.45 ± 0.1

6

7

pLp_0373sAmyb

5.3 ± 0.1

2.3 ± 0.1

30

8

pLp_0600sAmyb

1.8 ± 0.4

1.7 ± 0.2

49

9

pLp_3093sAmy

40.7 ± 2.0

0.30 ± 0.04

1

15

pLp_2958sAmyb

2.8 ± 0.8

1.5 ± 0.1

35

21

pUsp45sAmyb

29 ± 6

0.37 ± 0.14

1

23

pLp_2578sAmyb

2.4 ± 0.5

0.57 ± 0.2

19

27

pLp_2588sAmyb

2.0 ± 0.2

2.1 ± 0.1

51

30

pLp_2940sAmy

23.8 ± 6.4

(3.2 ± 0.3)d

n.d.d

41

pLp_3127sAmyb

28 ± 2

0.05 ± 0.01

0.2

56

pLp_1447sAmyb

1.5 ± 0.2

0.39 ± 0.08

21

57

pAmy-cyt

12.6 ± 4.6

0.05 ± 0.04

0

 
  1. a Plasmids encoding SPs are ranked according to their capacity to secrete NucA, as shown in Figure 1 (best SP first). All results are the mean value of three biological replicates.
  2. b Data from Mathiesen et al [19]. Note that the selection of SPs in this previous study was more or less random and not based on the genome-wide overview of SP functionality that is presented here.
  3. c Based on the assumption that all intracellular, non-processed AmyA is equally active as secreted AmyA.
  4. d Apparent secretion is partly due to cell lysis, meaning that the secretion efficiency could not be calculated. See text for details.
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BMC Genomics

ISSN: 1471-2164

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