Figure 2
Expression analysis of transgenic Arabidopsis plants that overexpress the CitrSEP gene. A, Semi-quantitative RT-PCR analysis of 12-day old untransformed Col-0 and transgenic lines L120-5 and L120-9. B, qRT-PCR of CitrSEP and endogenous SEP3 in transgenic lines. Expression was normalized to the expression of the constitutive EF-1-α gene, and then to the expression in Col-0 plants. For normalization purposes, the detection level of our qRT-PCR analysis was used as an estimate of CitrSEP expression in Col-0. Expression level is indicated in the plot. nd, not detected.