Gene array analysis of adrenal glands in broiler chickens following ACTH treatment

BMC Genomics

Table 2 Differential gene expression by microarray and RT-PCR analysis

	Microarray analysis		RT-PCR
Sequence	Fold H/C		Fold (H/C)	Fold (L/C)	Fold (H/L)
Genes up-regulated by ACTH treatment
ARL10	7	H = L > C	9	4	NS
ASAL	5.6	H = L > C	31	7	NS
PRAX-1	3.8	H = L > C	5	5	NS
C-TSK	3.4	H > L > C	57	14	4
ICER	3.4	H > C, C = L, L = H	12	NS	NS
HSD17B7	3.2	H > C, H > L, C = L	14	NS	4
STAR	2.3	H = L = C	NS	NS	NS
SC4MOL	1.5	H = L = C	NS	NS	NS
PISD	2.6	H = L > C	3	3	NS
ATF4	1.5	H = L = C	NS	NS	NS
FKBP	1.4	H = L = C	NS	NS	NS
Genes down-regulated by ACTH treatment
MYPN	3.2	H < L, H < C, L = C	15	7	NS
KCTD1	2.3	H < L, H < C, L = C	3	NS	3
NARFL	2	H = L = C	NS	NS	NS
NCB5R	1.4	H = L, H < C, L = C	2	NS	NS
Genes differentially expressed in high and low ACTH response groups
NUDT5	0.65	H = L = C	NS	NS	NS
AGT2	0.53	H = C = L, L > C	NS	2	NS
CCNG1	1.23	H = L = C	NS	NS	NS
PRAX-1	1.32	H = L, H > C, L > C	5	5	NS

ISSN: 1471-2164