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Table 2 Differential gene expression by microarray and RT-PCR analysis

From: Gene array analysis of adrenal glands in broiler chickens following ACTH treatment

  Microarray analysis   RT-PCR
Sequence Fold H/C   Fold (H/C) Fold (L/C) Fold (H/L)
Genes up-regulated by ACTH treatment
ARL10 7 H = L > C 9 4 NS
ASAL 5.6 H = L > C 31 7 NS
PRAX-1 3.8 H = L > C 5 5 NS
C-TSK 3.4 H > L > C 57 14 4
ICER 3.4 H > C, C = L, L = H 12 NS NS
HSD17B7 3.2 H > C, H > L, C = L 14 NS 4
STAR 2.3 H = L = C NS NS NS
SC4MOL 1.5 H = L = C NS NS NS
PISD 2.6 H = L > C 3 3 NS
ATF4 1.5 H = L = C NS NS NS
FKBP 1.4 H = L = C NS NS NS
Genes down-regulated by ACTH treatment
MYPN 3.2 H < L, H < C, L = C 15 7 NS
KCTD1 2.3 H < L, H < C, L = C 3 NS 3
NARFL 2 H = L = C NS NS NS
NCB5R 1.4 H = L, H < C, L = C 2 NS NS
Genes differentially expressed in high and low ACTH response groups
NUDT5 0.65 H = L = C NS NS NS
AGT2 0.53 H = C = L, L > C NS 2 NS
CCNG1 1.23 H = L = C NS NS NS
PRAX-1 1.32 H = L, H > C, L > C 5 5 NS
  1. NS: No statistical significant difference is shown between compared groups
  2. H: High responder group
  3. L: Low responder group
  4. C: Control group