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Table 1 Physiology in chemostat culture.

From: Transcriptomic comparison of Aspergillus niger growing on two different sugars reveals coordinated regulation of the secretory pathway

C-source

Strain

Cbiomass

(gDW kg-1)

Cxylose/maltose

(μM)

Yx/s

(gDW gsubstrate-1)

Yx/C

(gDWgcarbon-1)

qCO2

(mmol g-1 h-1)

qO2

(mmol g-1h-1)

RQ

qprotein-EC

(mg g-1 h-1)

C-recovery

(%)

Xylose

AB94-85

4.06 ± 0.07

233 ± 6

0.54 ± 0.02

1.35 ± 0.04

3.40 ± 0.11

3.36 ± 0.14

1.01 ± 0.02

0.68 ± 0.01

98 ± 2

 

ABGT1026

3.96 ± 0.07

256 ± 18

0.53 ± 0.01

1.32 ± 0.03

3.41 ± 0.10

3.42 ± 0.10

1.00 ± 0.01

0.72 ± 0.02

101 ± 3

Maltose

AB94-85

3.68 ± 0.08*

160 ± 5

0.52 ± 0.01

1.23 ± 0.03*

2.92 ± 0.05*

3.38 ± 0.14

0.87 ± 0.05*

1.98 ± 0.28*

101 ± 1

 

ABGT1026

3.68 ± 0.12*

158 ± 24

0.52 ± 0.02

1.23 ± 0.04*

2.84 ± 0.17*

3.57 ± 0.27

0.80 ± 0.10*

1.69 ± 0.24*

97 ± 2

  1. Steady state results of chemostat cultures with xylose or maltose as growth-limiting substrates. Standard deviations ( ± ) are given for mean values of triplicate independent steady state results. Cbiomass, dryweight biomass concentration; Cxylose/maltose, concentration of substrate (NB: maltose as glucose equivalents); Yxs, growth yield on substrate; YxC, growth yield on substrate carbon; qCO2, specific carbon dioxide evolution rate; qO2, specific oxygen consumption rate; RQ, respiratory quotient calculated as the ratio of CO2 production and O2 consumption rates; qprotein-EC, specific production rate of extracellular protein; C-recovery, carbon recovery. Two-tailed t-tests were used to evaluate significance (p < 0.05) of differences in each column (except substrate concentration and carbon recovery).
  2. * results are significantly different from unmarked results in the same column.
  3. † substrate concentration on maltose is given as concentration of glucose released into filtrate after incubation with α-glucosidase.