Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia

BMC Genomics

Table 2 Microarray and qRT-PCR analysis of selected genes showing differential expression in cepR, cciR or cepRcciIR mutants compared to K56-2.

Gene	Function^a	Change (fold) for K56-R2 (cepR) vs K56-2		Change (fold) for K56-2cciR vs K56-2		Change (fold) for K56-2cepRcciIR vs K56-2
		microarray^b	qRT-PCR^c	microarray^b	qRT-PCR^c	microarray^b	qRT-PCR^c
BCAS0409	zinc metalloprotease ZmpA	-5.3	-5.3	4.0	2.5	-4.5	-3.3
BCAL1814	MerR family regulatory protein	-2.9	-1.4	-2.7	-1.4	NC	2.7
BCAM0189	AraC family regulatory protein	-2.1	-1.3	NC	-2.6	NC	-3.4
BCAM0191	putative non-ribosomal peptide synthetase	-2.3	-7.4	NC	-2.1	NC	-6.8
BCAM0199	outer membrane efflux protein	2.1	1.6	NC	-1.5	NC	1.8
BCAS0293	nematocidal protein AidA	-88.7	-6647	NC	1.7	-214.9	-4632
BCAL0114	flagellin (type II) protein FliC	-2.4	-164.1	2.2	7.7	NC	-127.9
BCAS0225	Shiny variant regulator ShvR	-3.6	-2.6	NC	1.5	-2.3	-2.2
BCAS0220	putative permease	-3.0	-2.5	2.8	1.7	NC	-1.2
BCAS0204	ABC transporter ATP-binding protein	-7.4	-1.7	3.7	4.0	NC	-1.6
BCAM1420	RND family efflux system transporter protein	-4.8	-1.3	10.3	3.0	-7.4	-1.8
BCAM1418	two-component regulatory system, response	NC	-10.7	11.3	3.0	NC	-2.1
BCAM2626	heme receptor protein HuvA	3.4	2.2	-2.2	-2.0	2.7	2.6

^aFunction derived from B. cenocepacia J2315 [34].
^bChange in 16-h cultures of cepR, cciR or cepRcciIR mutants compared to 16-h cultures of K56-2 as determined by microarray analysis with at least two biological replicates (NC, no change).
^cChange in 16-h cultures of cepR, cciR or cepRcciIR mutants compared to 16-h cultures of K56-2 as determined by qRT-PCR.

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