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Table 5 Bacterial strains and plasmids used in this study.

From: Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia

Strain or plasmid

Description

Reference

Strains

  

B. cenocepacia

  

   K56-2

CF isolate, BCESM +,

[70]

   K56-R2

cepR::Tn5-OT182 derivative of K56-2, TcR

[14]

   K56-2ΔcepR

ΔcepR derivative of K56-2

This study

   K56-2cciR

cciR::Tp derivative of K56-2, TpR

[16]

   K56-2cciIR

ΔcciIR derivative of K56-2

[16]

   K56-2cepRcciIR

cepR::Tp, ΔcciIR derivative of K56-2, TpR

[16]

E. coli

  

   DH5α

F-mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 end A1 ara D139Δ(ara, leu)7697 gal U gal K λ-rps L nup G

Invitrogen

   TOP10

F-mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (StrR) end A1 nup G λ-

Invitrogen

   SY327

araD, Δ(lac pro) argE(Am) recA56 rifRnalA λpir

[71]

Plasmids

  

   pGPI-SceI

oriR6K, TpR, mob+, carries I-SceI cut site

[65]

   pDAI-SceI

pDA17 carrying the I-SceI gene

[65]

   TPCR2.1::H12

pCR2.1TOPO containing amplified homologous regions flanking cepR

This study

   pGPI-SceI::H12

Mutageneis plasmid pGPI-SceI with homologous regions flanking cepR

This study

   pAidA301

aidA::lux transcriptional fusion constructed in pMS402 KmR TpR

[28]

   pAfcA

afcA::lux transcriptional fusion constructed in pMS402 KmR TpR

This study