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Table 5 Bacterial strains and plasmids used in this study.

From: Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia

Strain or plasmid Description Reference
Strains   
B. cenocepacia   
   K56-2 CF isolate, BCESM +, [70]
   K56-R2 cepR::Tn5-OT182 derivative of K56-2, TcR [14]
   K56-2ΔcepR ΔcepR derivative of K56-2 This study
   K56-2cciR cciR::Tp derivative of K56-2, TpR [16]
   K56-2cciIR ΔcciIR derivative of K56-2 [16]
   K56-2cepRcciIR cepR::Tp, ΔcciIR derivative of K56-2, TpR [16]
E. coli   
   DH5α F-mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 end A1 ara D139Δ(ara, leu)7697 gal U gal K λ-rps L nup G Invitrogen
   TOP10 F-mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (StrR) end A1 nup G λ- Invitrogen
   SY327 araD, Δ(lac pro) argE(Am) recA56 rifRnalA λpir [71]
Plasmids   
   pGPI-SceI oriR6K, TpR, mob+, carries I-SceI cut site [65]
   pDAI-SceI pDA17 carrying the I-SceI gene [65]
   TPCR2.1::H12 pCR2.1TOPO containing amplified homologous regions flanking cepR This study
   pGPI-SceI::H12 Mutageneis plasmid pGPI-SceI with homologous regions flanking cepR This study
   pAidA301 aidA::lux transcriptional fusion constructed in pMS402 KmR TpR [28]
   pAfcA afcA::lux transcriptional fusion constructed in pMS402 KmR TpR This study