From: Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia
Strain or plasmid | Description | Reference |
---|---|---|
Strains | Â | Â |
B. cenocepacia | Â | Â |
   K56-2 | CF isolate, BCESM +, | [70] |
   K56-R2 | cepR::Tn5-OT182 derivative of K56-2, TcR | [14] |
   K56-2ΔcepR | ΔcepR derivative of K56-2 | This study |
   K56-2cciR | cciR::Tp derivative of K56-2, TpR | [16] |
   K56-2cciIR | ΔcciIR derivative of K56-2 | [16] |
   K56-2cepRcciIR | cepR::Tp, ΔcciIR derivative of K56-2, TpR | [16] |
E. coli | Â | Â |
   DH5α | F-mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 end A1 ara D139Δ(ara, leu)7697 gal U gal K λ-rps L nup G | Invitrogen |
   TOP10 | F-mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (StrR) end A1 nup G λ- | Invitrogen |
   SY327 | araD, Δ(lac pro) argE(Am) recA56 rifRnalA λpir | [71] |
Plasmids | Â | Â |
   pGPI-SceI | oriR6K, TpR, mob+, carries I-SceI cut site | [65] |
   pDAI-SceI | pDA17 carrying the I-SceI gene | [65] |
   TPCR2.1::H12 | pCR2.1TOPO containing amplified homologous regions flanking cepR | This study |
   pGPI-SceI::H12 | Mutageneis plasmid pGPI-SceI with homologous regions flanking cepR | This study |
   pAidA301 | aidA::lux transcriptional fusion constructed in pMS402 KmR TpR | [28] |
   pAfcA | afcA::lux transcriptional fusion constructed in pMS402 KmR TpR | This study |