Results of secondary structure predictions by SignalP and TMHMM. A) A signal peptide at N-terminus (green), a PEXEL motif (light blue) and two transmembrane helices (TM1 and TM2; dark green) were predicted for RIF_Bs. A sequence logo for TM1 is also shown to highlight a pattern of conserved glycines. B) No signal peptide was predicted for the majority of RIF_As, and only one C-terminal transmembrane helix was found (TM; dark green). Insert (25 aa long) is indicated in red and corresponding sequence logo is also shown. In both RIF_As and RIF_Bs, the region between the PEXEL and the insert is highlighted (yellow). This region is conserved among all members of RIFIN family.