Figure 3

Acute systemic administration of formoterol alters the expression of genes associated with metabolism. Quantitative RT-PCR was used to assay the expression of A. Hk2, B. Pdk4, C. Sorbs1, D. Pgc1α, E.Lipin1α, F. FoxO1, and G.Ucp3 mRNAs in tibialis anterior over acute timepoints. Muscles were removed at 1, 4, 8 and 24 hours following a single intraperitoneal injection of formoterol or saline vehicle (NT = no treatment). Results were normalized against 36B4 at each timepoint. Statistical significance was assessed using a one-way ANOVA with Bonferroni's post-test where p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***). Unmarked data points are non-significant. G. Diagrammatic representation of acute gene expression changes related to metabolic in skeletal muscle in response to formoterol.