Figure 5

Acute and chronic systemic administration of formoterol alters the expression of genes associated with circadian rhythm and transcriptional regulation. Quantitative RT-PCR was used to assay the expression of A. Nfil3, B. Dbp, C. Nurr1, D. Creb, and E.Cebpb mRNAs in tibialis anterior over acute timepoints. Muscles were removed at 1, 4, 8 and 24 hours following a single intraperitoneal injection of formoterol or saline vehicle (NT = no treatment). For chronic timepoints, the expression of F. Nfil3, G. Dbp, H. Nurr1, I. Creb, and J.Cebpb mRNAs were measured in tibialis anterior removed at 1, 7 and 28 days following daily intraperitoneal injection of formoterol or saline vehicle (NT = no treatment). All results were normalized against 36B4 at each timepoint. Data are expressed as mean ± SEM (n = 5). Statistical significance was assessed using a one-way ANOVA with Bonferroni's post-test where p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***). Unmarked data points are non-significant.