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Table 1 Statistics of small RNA sequences from the Brachypodium NC and WC libraries.

From: Deep sequencing of Brachypodium small RNAs at the global genome level identifies microRNAs involved in cold stress response

 

Sequences generated

Unique sequences

NC library

  

   Raw reads

5,339,385

 

   Low quality reads removed

4,702,002

 

   Adaptors removed

3,816,932

 

   Sequences < 18 nt removed

3,741,194

 

   Sequences matching Brachypodium draft genome

2,603,872

 

   Non-coding RNA exact matches removed

2,248,474

711,207

   Match known miRNAs

851,945

27

   Genome-matched small RNAs with predicted hairpin structures

41,155

235

WC library

  

   Raw reads

5,452,742

 

   Low quality reads removed

4,720,226

 

   Adaptors removed

4,314,516

 

   Sequences < 18 nt removed

3,585,498

 

   Sequences matching Brachypodium draft genome

2,958,093

 

   Non-coding RNA exact matches removed

1,264,919

423,463

   Match known miRNAs

337,863

27

   Genome-matched small RNAs with predicted hairpin structures

12,750

218