Profiling of miRNA expression during pupal metamorphosis. (A) Developmental time-points were grouped using the hierarchical clustering method and gene sets from Additional file 9. Gene names and quantitative miRNA expression levels are presented in Additional file 9. (B) Northern blotting analysis of specific miRNAs in females. (C) Stage-regulated miRNAs in females. (D) Stage-regulated miRNAs in males. (E) Northern blotting analysis of specific miRNAs in males. Northern blots were exposed for different times, and thus the intensities of signals on one Northern blot cannot be directly compared to those from others. A probe against 5srRNA was hybridized to respective blots for comparison.