Figure 7
Differential expression of the COG0523 genes of Chlamydomonas reinhardtii. Chlamydomonas strain 2137 was grown under various metal-deficient or replete conditions in triplicate experiments, represented by squares, circles and triangles. RNA was isolated from these cultures and analyzed by real time PCR. Each metal deficiency, zinc, copper, iron, and manganese, is shown in a separate panel. RNA abundance is expressed relative to the metal-replete condition. Each data point represents an independent experiment with each measurement representing the average of technical triplicates. CBLP was used as the reference gene. ZRT3, CYC6, FOX1 and NRAMP1 served as positive controls for Zn-, Cu-, Fe-, and Mn-deficiency, respectively. (Note that Cu-deficiency is a secondary effect of Zn-deficiency (Malasarn, unpublished) and Fe-deficiency is a secondary effect of Mn-deficiency [92].)