Figure 3

Close correspondence of CO/NCO loci to hotspots for double stranded breaks (DSBs). We examined DSB densities in 20-kb regions flanking each locus in different sets of COs/NCOs from our studies and those reported by Mancera et al. [12]; Genome-wide DSB density data from 40,000 probes were obtained from Buhler et al. [19], and average densities for 1-kb intervals (centered at the position shown on the X-axis) in 10-kb regions upstream and downstream regions flanking each locus in a specific set of COs or NCOs. The yellow (square) and green (diamond) lines are for the 91 COs and 21 GCs from this study, respectively, whereas the purple (triangle) and blue (circle) lines are for the 4,394 COs and 2,397 NCOs, respectively, in the Mancera et al. study [12]. All four datasets show a clear correspondence of CO/NCO sites to the highest average DSB densities, suggesting that they are enriched for DSB hotspots. The grey line represents the DSB densities of 20-kb regions around 5,000 random sites, as a negative control. The two ovals at the top indicate the estimated average length of COs/NCOs from our study.