Characterization of meiotic crossovers and gene conversion by whole-genome sequencing in Saccharomyces cerevisiae

Table 2 Coverage of S288C by assembled contigs based on combined reads from different spores

Template	coverage	# contigs	Largest contig (bp)	Ave. contig size (bp)	Median contig size (bp)	N50 contig size (bp)	Assembled size (Mb)	# assembled reads	% of Reference
Spore 1	4.6 ×	12,272	8,128	768	589	1,019	9.4	302,707	79%
Spore 2	4.9 ×	9,683	9,655	1,053	779	1,505	10.2	306,022	85%
Spore 3	4.7 ×	10,281	9,586	959	721	1,323	9.9	263,286	83%
Spore 4	3.6 ×	21,513	9,081	290	254	308	6.2	242,053	40%
2 spores (1+2)	9.5 ×	3,158	42,872	3,586	2,069	7,242	11.3	644,429	94%
2 spores (1+3)	9.3 ×	3,369	34,567	3,348	1,973	6,593	11.3	601,938	94%
2 spores (2+3)	9.6 ×	2,749	38,236	4,132	2,375	8,535	11.4	594,797	94%
3 spores (1+2+3)	14.2 ×	1,836	79,085	6,235	1,560	18,824	11.4	906,455	95%
4 spores	17.8 ×	7,730	91,399	1,686	265	14,948	13.0	1,224,321	95%

Software NEWBLER (Roche off-instrument software) was used for assembly of different datasets. Contigs homologous to mitochondrial sequence of S288C were removed before further analysis.

ISSN: 1471-2164