Figure 3

Androgen regulation of genes as measured by qRT-PCR. A Candidate genes not previously implicated to change expression in response to androgens in prostate cancer cells, and B Genes known to change levels of expression in response to androgens. LNCaP cells were treated for 16 hours prior to harvesting RNA, and analysing mRNA levels by qRT-PCR. Fold-change was calculated by normalizing the mean normalized expression (MNE) of transcripts in R1881-treated cells to the mock vehicle-treated cells. In doing this, the vehicle treatment fold-change became one and standard deviation (SD) zero. Error bars represent ± SD for biological sextuplets. [*] Asterisk indicates significant differential gene expression according to the Two-Sample Student's T-test (p ≤ 0.05) for unequal variance.