Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring

Table 6 Validation of microarray data with stem-loop real-time qPCR

Genes	qPCR (arbitrary units)		Microarray		↓ Fold (HF/C v C/C)
	C/C (n = 7)	HF/C (n = 7)	C/C	HF/C	qPCR	Microarray
*Stem-loop real time PCR*
miR-30c	1572 ± 670	1168 ± 230*	19436	18861	1.35	1.03
miR-22	309 ± 163	440 ± 179*	130448	44304	0.70	2.94
miR-29a	370 ± 117	276 ± 109*	30002	16331	1.34	1.84
Let-7c	330 ± 31	227 ± 19*	108628	71343	1.45	1.52
miR-483*	1.9 ± 0.2	0.3 ± 0.1***	366458	74387	6.89	4.93
*Poly dT adaptor qPCR method*
miR-709	1304 ± 118	768 ± 129**	1387121	1258883	1.70	1.10
miR-122	948 ± 61	473 ± 40***	324798	192912	2.00	1.68
miR-192	321 ± 24	121 ± 10***	80361	44865	2.64	1.79
miR-194	124 ± 11	81 ± 7**	92178	49817	1.53	1.85
miR-26a	157 ± 17	58 ± 10***	95209	58142	2.72	1.64
Let-7c	118 ± 10	60 ± 7***	108628	71343	1.96	1.52
let-7a	279 ± 16	108 ± 12***	60650	43004	2.59	1.41
let-7b	107 ± 5	46 ± 4***	83730	63907	2.34	1.31
let-7d	165 ± 16	80 ± 8***	91702	72642	2.05	1.26
miR-494	12 ± 2	5.5 ± 0.8***	298733	82842	2.18	3.61
miR-483	1.3 ± 0.2	1.0 ± 0.2	17689	22100	0.78	0.80

Levels of miRNAs were measured either with purchased primers for specific miRNAs (stem-loop real time PCR) or poly dT adaptor as described in the method section. Mean ± S.E. * p < 0.05 and *** p < 0.001 (HF/C v C/C). ** p < 0.01, and ***p < 0.001 (HF/C v C/C).

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