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Table 1 Summary of DNA sequence filtering results.

From: Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey

 

Filter applied1

Pass Filter

(%)

GATC start (%)

Pre-selection

l32 n.

107888201

94.48

24.78

Assembly

l32 n. q20 o230

27979963

24.50

46.64

SNP

l32 n. q10 o230

32941906

28.84

40.23

  1. 1 sequences are filtered for length 32, without base-call errors (n or .). Singly represented reads are required to have a per base-call quality score of 20 (assembly data set) or 10 (SNP data set). Sequences more than four times overrepresented, based on the expected 56× coverage, were discarded.