Deduced α-gliadin isoforms and CD-epitope frequencies of cultivars. Amino acid sequences of the N-terminal part of α-gliadins as deduced from α-gliadin transcripts present in developing grains at 14 days post anthesis (14DPA) of single genotypes derived from the respective cultivars 'Lavett' (3A), 'Baldus' (3B), This N-terminal locus contains several CD-epitopes: p31-49 (PGQQ QPFPP Q QP YPQPQPF, in blue), triggering the innate immune response; and the HLA-DQ2+ restricted T-cell epitopes Glia-α9(αI) (PFPQPQ LPY, in red); Glia-α9(αIII) (PYPQPQ LPY); Glia-α2(αII) (PQPQ LPYPQ) and Glia-α20 (FRPQ QPYPQ, in green). The HLA-DQ2+ epitope frequency calculated as the number of conserved (ESTs) for 'Lavett', 'Baldus' is respectively 2.7 and 1.6. The consensus nucleotide sequences of the EST contigs were assigned to a predicted genomic location by clustering (Clustal W) with genomic sequences from diploid species with genome AA (T. monococcum), BB (SS) (Aegilops speltoides) and DD (Aegilops tauschii), as in Van Herpen et al. . GenBank accession numbers of the ESTs: 'Lavett' GH160345-GH160365, 'Baldus' GH162284-GH160344.