Gli-A2 transcript frequency in different wheat genotypes. The Gli-A2 frequency in the total α-gliadin transcriptome of developing seeds (cDNA). Among the accessions are advanced cultivars of bread wheat (T. aestivum L.) (in white), a durum wheat (T. turgidum var. durum) cultivar (in yellow), an accession used for breeding research (CGN10567) and more genetically diverse landraces (in purple). For each accession, the Gli-A2 frequency in the cDNA fractions of two individual plants was measured with pyrosequencing on two SNP positions C2/C2+T3 and G20/G20+A21. Bars indicate the standard deviation for the technical replications. The advanced cultivars are either registered as tetraploid (4×) or hexaploids (6×). Landraces (blue bars) may be mixtures of both ploidy levels and therefore the ploidy level of the individuals derived from a landrace accession is uncertain. More information about the accessions is given in Table 1.