Skip to main content

Table 2 Gli-A2 specific pyrosequencing in genomic DNA of 'Chinese Spring'

From: Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

Line SNP1 (T2C3) mean stdev n SNP2 (G20A21) mean stdev n SNP1+SNP2 average Differences
P < 0.001
WT4086 0.16 0.04 5 0.20 0.02 5 0.181 a
WT4085 0.16 0.05 5 0.21 0.03 5 0.185 a
WT4084 0.15 0.05 8 0.18 0.03 8 0.165 a
6AS-1 0.00 0.02 5 0.00 0.01 5 0.000 b
  1. Frequency of α-gliadin gene copies derived from the Gli-A2 locus in genomic DNA of three 'Chinese Spring' accessions and in the homozygous deletion line 6AS-1 of 'Chinese Spring' missing the short arm of chromosome 6A, as determined by pyrosequencing on two SNP positions (T2/C3 & GG20/A21), n = number of technical repetitions performed per 'Chinese Spring' line.; a, b: a different letter indicates a statistically significant difference between the two samples; LSD for the Gli-A2 frequency among 'Chinese Spring' lines = 3.3% ± 0.004 at P < 0.001 (general ANOVA; Genstat).