Line | SNP1 (T2C3) mean | stdev | n | SNP2 (G20A21) mean | stdev | n | SNP1+SNP2 average | Differences P < 0.001 |
|---|
WT4086
| 0.16 | 0.04 | 5 | 0.20 | 0.02 | 5 | 0.181 | a |
WT4085
| 0.16 | 0.05 | 5 | 0.21 | 0.03 | 5 | 0.185 | a |
WT4084
| 0.15 | 0.05 | 8 | 0.18 | 0.03 | 8 | 0.165 | a |
6AS-1
| 0.00 | 0.02 | 5 | 0.00 | 0.01 | 5 | 0.000 | b |
- Frequency of α-gliadin gene copies derived from the Gli-A2 locus in genomic DNA of three 'Chinese Spring' accessions and in the homozygous deletion line 6AS-1 of 'Chinese Spring' missing the short arm of chromosome 6A, as determined by pyrosequencing on two SNP positions (T2/C3 & GG20/A21), n = number of technical repetitions performed per 'Chinese Spring' line.; a, b: a different letter indicates a statistically significant difference between the two samples; LSD for the Gli-A2 frequency among 'Chinese Spring' lines = 3.3% ± 0.004 at P < 0.001 (general ANOVA; Genstat).
