|
Line
|
SNP1 (T2C3) mean
|
stdev
|
n
|
SNP2 (G20A21) mean
|
stdev
|
n
|
SNP1+SNP2 average
|
Differences
P < 0.001
|
|---|
|
WT4086
|
0.16
|
0.04
|
5
|
0.20
|
0.02
|
5
|
0.181
|
a
|
|
WT4085
|
0.16
|
0.05
|
5
|
0.21
|
0.03
|
5
|
0.185
|
a
|
|
WT4084
|
0.15
|
0.05
|
8
|
0.18
|
0.03
|
8
|
0.165
|
a
|
|
6AS-1
|
0.00
|
0.02
|
5
|
0.00
|
0.01
|
5
|
0.000
|
b
|
- Frequency of α-gliadin gene copies derived from the Gli-A2 locus in genomic DNA of three 'Chinese Spring' accessions and in the homozygous deletion line 6AS-1 of 'Chinese Spring' missing the short arm of chromosome 6A, as determined by pyrosequencing on two SNP positions (T2/C3 & GG20/A21), n = number of technical repetitions performed per 'Chinese Spring' line.; a, b: a different letter indicates a statistically significant difference between the two samples; LSD for the Gli-A2 frequency among 'Chinese Spring' lines = 3.3% ± 0.004 at P < 0.001 (general ANOVA; Genstat).
