From: Characterization of the oxidative stress stimulon and PerR regulon of Campylobacter jejuni
Strain or plasmid | Relevant characteristics a | Source or reference |
---|---|---|
E. coli strains | Â | Â |
   DH5α | end A1, hsd R17 (rK-, mK-), sup E44, thi-1, rec A1, gyr A, rel A1, Δ(lac ZYAarg F)U169, deo R, [f80dlac Δ(lacZ0 M15] | Invitrogen |
   E. coli K-12 | end A1, hsd R17 (rK12 -, mK12 +), sup E44, th i-1, rec A1, gyr A96, rel A1, lac F' [proA+B+, lacIqZDM 15::Tn 10(tetR)] | BD Fusion-blue competent cells |
C. jejuni strains | Â | Â |
   NCTC 11168 | C. jejuni NCTC 11168 | NCTC |
   AS230 | NCTC 11168Δfur | [24] |
   AS216 | NCTC 11168ΔperR | This study |
   AS232 | NCTC 11168Δfur ΔperR | This study |
   AS433 | NCTC 11168ΔkatA | This study |
   AS438 | NCTC 11168ΔahpC | This study |
   AS446 | NCTC 11168ΔsodB | This study |
   AS960 | NCTC 11168Δfur+fur | This study |
   AS978 | NCTC 11168ΔkatA+katA | This study |
   AS1016 | NCTC 11168ΔahpC+ahpC | This study |
   AS990 | NCTC 11168ΔsodB+sodB | This study |
   AS1038 | NCTC 11168ΔperR+perR | This study |
Plasmids | Â | Â |
   pUC19 | Cloning and suicide vector, Ampr | Biolabs |
   pILL600 | Plasmid carrying kan |  |
   pRR | Fragment of rRNA gene cluster cloned into pGEM-T Easy | B. Wren |
   pRRC | cam cassette cloned into pRR | B. Wren |
   pRRK | kan cassette cloned into pRR | This study |
   pAS214 | pUC19 carrying ΔperR::cam | This study |
   pAS215 | pUC19 carrying ΔperR::kan | This study |
   pAS431 | pUC19 carrying ΔkatA::cam | This study |
   pAS436 | pUC19 carrying ΔahpC::kan | This study |
   pAS445 | pUC19 carrying ΔsodB::cam | This study |
   pAS930 | pRRK carrying the fur gene | This study |
   pAS946 | pRRK carrying the katA gene | This study |
   pAS1010 | pRRC carrying the ahpC gene | This study |
   pAS985 | pRRK carrying the sodB gene | This study |