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Table 3 Bacterial strains and plasmids used in this study.

From: Characterization of the oxidative stress stimulon and PerR regulon of Campylobacter jejuni

Strain or plasmid

Relevant characteristics a

Source or reference

E. coli strains

  

   DH5α

end A1, hsd R17 (rK-, mK-), sup E44, thi-1, rec A1, gyr A, rel A1, Δ(lac ZYAarg F)U169, deo R, [f80dlac Δ(lacZ0 M15]

Invitrogen

   E. coli K-12

end A1, hsd R17 (rK12 -, mK12 +), sup E44, th i-1, rec A1, gyr A96, rel A1, lac F' [proA+B+, lacIqZDM 15::Tn 10(tetR)]

BD Fusion-blue competent cells

C. jejuni strains

  

   NCTC 11168

C. jejuni NCTC 11168

NCTC

   AS230

NCTC 11168Δfur

[24]

   AS216

NCTC 11168ΔperR

This study

   AS232

NCTC 11168Δfur ΔperR

This study

   AS433

NCTC 11168ΔkatA

This study

   AS438

NCTC 11168ΔahpC

This study

   AS446

NCTC 11168ΔsodB

This study

   AS960

NCTC 11168Δfur+fur

This study

   AS978

NCTC 11168ΔkatA+katA

This study

   AS1016

NCTC 11168ΔahpC+ahpC

This study

   AS990

NCTC 11168ΔsodB+sodB

This study

   AS1038

NCTC 11168ΔperR+perR

This study

Plasmids

  

   pUC19

Cloning and suicide vector, Ampr

Biolabs

   pILL600

Plasmid carrying kan

 

   pRR

Fragment of rRNA gene cluster cloned into pGEM-T Easy

B. Wren

   pRRC

cam cassette cloned into pRR

B. Wren

   pRRK

kan cassette cloned into pRR

This study

   pAS214

pUC19 carrying ΔperR::cam

This study

   pAS215

pUC19 carrying ΔperR::kan

This study

   pAS431

pUC19 carrying ΔkatA::cam

This study

   pAS436

pUC19 carrying ΔahpC::kan

This study

   pAS445

pUC19 carrying ΔsodB::cam

This study

   pAS930

pRRK carrying the fur gene

This study

   pAS946

pRRK carrying the katA gene

This study

   pAS1010

pRRC carrying the ahpC gene

This study

   pAS985

pRRK carrying the sodB gene

This study

  1. acam, chloramphenicol resistance gene; Ampr, ampicillin resistant; kan, kanamycin resistance gene.