Table 4 Primers used in this study.
From: Characterization of the oxidative stress stimulon and PerR regulon of Campylobacter jejuni
Primer | DNA sequence from 5' to 3' |
|---|---|
For gene cloning | |
   perR-01 | ATGCAGATCT GTTATGGACAAGGTGTGGCA (BglII) |
   perR-02 | ATGCAGATCT CATTGGAACTATCCAAAGTTGG (BglII) |
   katA-01 | ATGCGGTACC CGATTTTGGAAACATTATAGCTGA (KpnI) |
   katA-02 | ATGCCTGCAG TAATGGGGTTTGTCCACGAT (PstI) |
   ahpC-01 | ATGCAGATCT CTGGAGTGTCCCCACTTCTC (BglII) |
   ahpC-02 | ATGCAGATCT CCAAAACCCCAGCAATCATA (BglII) |
   sodB-01 | ATGCGAATTC TTAAAAGTGGCCCATTCGTC (EcoRI) |
   sodB-02 | ATGCCTGCAG TCCTTTTACTTCACGCAAGC (PstI) |
For inverse PCR | |
   perR-03 | ATGCGGATCC CACATAGTCTTTGCGGAGTAGC (BamHI) |
   perR-04 | ATGCGGATCC GATAGGCAATCTCGTCAATCA (BamHI) |
   katA-03 | ATGCGGATCC ATAAGCTCGGCAGCTTCTTG (BamHI) |
   katA-04 | ATGCGGATCC ACAATGTCGCTGGTGCTATG (BamHI) |
   ahpC-03 | ATGCGGATCC ACCGCTCCTTTTGGACCTAT (BamHI) |
   ahpC-04 | ATGCGGATCC TCGCCATGCTGTGGTTAAT (BamHI) |
   sodB-03 | ATGCGGATCC TCACCAAAAGCATTGGTATCAT (BamHI) |
   sodB-04 | ATGCGGATCC GGCATGGGATCAGTTAGCTT (BamHI) |
For complementation | |
   aphA3-01 | TCCCCCGGG GATAAACCCAGCGAAC (SmaI) |
   aphA3-02 | TCCCCCGGG AAGCTTTCTAGA CATCTAAATC (SmaI, XbaI) |
   fur-05 | GATTTAGATGTCTAGCATGCTAGTGAAAAGTTGCAAGA |
   fur-06 | GGGGAAGCTTTCTAGGCTTTTTCTATTCTTTGCTGCTC |
   katA-05 | GATTTAGATGTCTAGTTACGTGCATCCCAGTGTTC |
   katA-06 | GGGGAAGCTTTCTAGCCACCAAAAGTGGCAAGTAAA |
   ahpC-05 | ATCCACTAGTTCTAGCATTCAACGCATTTGTTTGC |
   ahpC-06 | CTAGGGCCGCTCTAGAAAAATGAAGAAAAAGCCTCGT |
   sodB-05 | GATTTAGATGTCTAGTTTTTCATATTTTCCTCCTTATGAA |
   sodB-06 | GGGGAAGCTTTCTAGCATTTTTGCCCCTTTTTGTG |
   perR-05 | GATTTAGATGTCTAGGACCTATTGCTTTGCGTTAT |
   perR-06 | GGGGAAGCTTTCTAGTGCTTAATGACACTTTTTGC |
For identification of the rRNA insertion site | |
   Ak233 | GCAAGAGTTTTGCTTATGTTAGCAC |
   Ak234 | GAAATGGGCAGAGTGTATTCTCCG |
   Ak235 | GTGCGGATAATGTTGTTTCTG |
   AR56 | CATCCTCTTCGTCTTGGTAGC |
   Ak237 | TCCTGAACTCTTCATGTCGATTG |