Figure 1

Controls for qRT²-PCR. Polyacrylamide gel electrophoresis of the products from 30 cycles of real-time PCR on cDNA derived from PK-15 cells infected by the PRV for 1, 2, 4 or 6 h or from cells infected by the virus for 4 or 6 h and treated with CHX or PAA. A no-template control was used for each primer pair, consisting of water, to test for false-positive results, and no-RT control was used to ensure the absence of DNA contamination. The GeneRuler™ Low Range DNA Ladder (Fermentas) is shown at the left side of the photo. The cDNAs of ie180 gene (a) and ul30 gene (b) were used for the presentation.