Figure 3
From: Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay
Effects of the CHX on the expression of ie180 , llt1 , llt2 and ep0 genes. This Figure shows the dependence of the PRV gene expression on de novo protein synthesis on two genes (ie180 and ep0) and two antisense transcripts (llt1 and llt2) overlapping these genes. PK-15 cells infected with PRV were incubated in the presence or absence of CHX for 2, 4 or 6 h. The CHX-mediated inhibition of viral gene expression (Ri-CHX) was calculated as follows: Ri-CHX = RCHX/RUT, where RCHXis the R value of the CHX-treated samples; and RUTis the R value of the untreated samples.