Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay

Tombácz, Dóra; Tóth, Judit S; Petrovszki, Pál; Boldogkői, Zsolt

doi:10.1186/1471-2164-10-491

BMC Genomics

Table 1 Function and kinetic grouping of PRV genes

From: Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay

Name			Transcription Kinetics					Function(s)^b	References
	Wagner³⁷	Wagner³⁸	Roizman³⁹	Roizman⁴⁰	Mettenleiter⁴¹	Mettenleiter⁴¹	Our data^a
orf-1	-	-	-	-	-	ND	L	unknown
ul54 (ICP27)*	IE	IE	IE	IE	IE	E	E	transcription regulation	[40]
ul53 (gK)	L	EL	L	L	L	(EL) 3 h pi	E/L	viral egress	[40]
ul52	E	E	E	E	E	(E) 2 h pi	E	DNA replication	[40]
ul51	L	EL	L	L	L	E	L	viral egress/oralgen unknown	[40]
ul50 (dUTPase)*	E	E	E	E	E	(EL) 3 h pi	E	dUTPase, viral replication	[27]
ul49.5 (gN)	L	L	L2	L2	L	L	L	virion entry	[39]
ul49 (VP22)*	E?	E	L	L	E	(EL) 4 h pi	L	virion formation, tegumentation	[27]
ul48 (VP16, IE-TIF)*	?	EL	L	L	L	(L) 8 h pi	L	gene regulation, viral egress	[41]
ul47 (VP13/14)*	E	EL	L2	L2	L	L	L	secondary envelopment	[41]
ul46 (VP11/12)*	E	EL	L	L	L	E	E/L	unknown function, tegument protein	[41]
ul27 (gB)	E	EL	L1	L1	E	ND	L	cell-cell spread, virus entry	[27]
ul28 (ICP18.5)*	E	EL	L	L	E	E	E	DNA cleavage and packaging	[27]
ul29 (ICP8)*	E	E	E	E	ND	ND	E	DNA replication	[27]
ul30	E	E	E	L	E	ND	E	DNA replication	[27]
ul31	L	EL	L2	L2	L	(L) 6 h pi	L	nuclear egress	[42]
ul32	L	EL	L2	L2	L	ND	L	DNA packaging	[2]
ul33	L	EL	?	L	L	ND	L	encapsidation of viral DNA	[43]
ul34	L	L	?	L	ND	(EL) 3 h pi	L	nuclear egress	[14]
ul35 (VP26)*	L	L	L2	L2	L	ND	L	capsid protein	[44]
ul36 (VP1/2)*	?	EL	L2	L2	L	(EL) 3-9 h pi	L	tegumentation and egress	[45]
ul37	L	E	L	L	L	(E) 2 h pi	L	secondary envelopement, egress	[45]
ul38 (VP19c*)	L	L	L2	L2	L	(E) 2 h pi	L	capsid protein	[46]
ul39 (RR1)	E	E	E	E	E	(E) E/L	E/L	nucleotide synthesis	[46]
ul40 (RR2)	E	E	E	E	E	E	E	nucleotide synthesis	[47]
ul41 (VHS)	L	EL	L	L	L	ND	L	RNase, gene regulation	[48]
ul42	L	E	E	E	E	L	L	DNA replication	[47]
ul43	L	EL	?	L	E	E	E/L	unknown	[49]
ul44 (gC)	L	L	L2	L2	L	L	L	viral entry, virion attachment	[50]
ul26	E	L	L	L	L	L	L	scaffold protease	[52]
ul25	L	EL	L	L	L	L	L	capsid protein	[51]
ul24 (VP24)*	L	L	L	L	L	L	L	unknown	[52]
ul23 (TK)	E	E	E	E	E	E	E	nucleotide synthesis	[47]
ul22 (gH)	L	L	L2	L2	L	L	L	viral entry, cell-cell spread	[47]
ul21	L	E	?	L	L	L	E	capsid maturation	[47]
ul20	L	EL	L	L	L	ND	E/L	capsid transport	[2]
ul19 (VP5)*	L	EL	L1	L1	L	(L) 16 h pi	L	capsid protein	[53]
ul18 (VP23)*	L	EL	L	L	L	ND	L	capsid protein	[27]
ul17	L	EL	L	L	L	ND	L	DNA cleavage and encapsidation	[2]
ul16	?	EL	?	L	L	ND	L	unknown, interacts with UL11	[27]
ul15	L	EL	L	L	L	ND	E/L	DNA cleavage and encapsidation	[27]
ul14	L	EL	?	L	L	L	E/L	DNA cleavage and packaging	[47]
ul13 (VP18.8)*	L	EL	L	L	L	(E) EL	E	protein phosphorylation	[47]
ul12 (AN)*	E	E	E	E	E	(E) EL	E	alkaline nuclease	[47]
ul11	L	EL	L(?)	L	L	L	E	secondary envelopement	[47]
ul10 (gM)	L	E	L	L	L	E	L	egress, secondary envelopement	[54]
ul9 (OBP)*	E	E	L(?)	E	E	E	E	ori depentent DNA synthesis	[54]
ul8 (OBPC)*	E	E	E	E	E	(E/L) 3-5 h pi	E	DNA replication	[54]
ul7	?E	E	?	ND	L	(E/L) dE	E/L	unknown	[54]
ul6	L	E	?	ND	L	(E/L) dE	L	capsid protein, portal protein	[54]
ul5	E	E	E	E	E	(L) 6 h pi	L	DNA replication	[55]
ul4	E	E	?	L	L	(L) 6 h pi	E/L	unknown	[55]
ul3.5	-	-	-	-	-	E/L	L	replication, cell-to-cell spread	[55]
ul3	L	L	L2	L2	L	E/L	L	unknown	[55]
ul2 (UNG)*	E	E	E	L	E	E/L	L	DNA repair	[55]
ul1 (gL)	E	EL1	L	L	L	E/L	L	viral entry, cell-to-cell spread	[55]
ep0 (ICP0)*	IE	IE	IE	IE	E	E	E	gene regulation	[10]
llt1	L	ND	ND	ND	ND	ND	L	latency	[56]
llt2	L	ND	ND	ND	ND	ND	E	latency	[56]
ie180 (ICP4)*	IE	IE	IE	IE	IE	IE	E/L	gene regulation	[13]
us1 (RSp40/ICP22)*	IE	IE	IE	IE	IE	IE	L	regulator of gene expression	[27]
us3(PK)	E	E	E	E/L	E	E	E	nuclear egress	[27]
us4(gG)	E	E	L	L	E/L	E	E/L	unknown	[57]
us6 (gD)	L	E	L1	E/L	E/L	ND	E/L	entry	[27]
us7 (gI)	E	E	L	L	L	(L) 6 h pi	E/L	cell-to-cell spread	[27]
us8 (gE)	E	E	L2	L2	L	E	E/L	cell-to-cell spread	[58]
us9 (*11K)	E	E	?	L	L	L	E/L	anterograde spreed of virus	[58]
us2 (*28K)	E	E	?	L	L	(L) 5 h pi	E/L	unknown	[27]

This Table demonstrates the function and kinetic classification of PRV genes in comparison with available data on HSV and PRV genes.
^a PRV genes were classified on the bases of the ratio of the PAA-treated and untreated samples, at 6 h pi.
^b Gene functions in italics rely on studies of the HSV-1 homologs.

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