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Figure 2 | BMC Genomics

Figure 2

From: Quality control in microarray assessment of gene expression in human airway epithelium

Figure 2

Assessment of GAPDH 3'/5' and Chip scaling factor. Ratios of signal intensities for GAPDH 3' and 5' probe sets for 223 samples were extracted from the GeneChip Operating Software (GCOS) Quality Report and plotted against the Scaling Factors analyzed with a target intensity value of 500 extracted from the GCOS Quality Report. Samples with GAPDH 3'/5' ratio ≤ 3.0, to the left of the vertical dotted line, passed QC criterion, while the one sample to the right of the dotted line failed the QC criterion. Samples with scaling factor values ≤ 10.0 passed QC criterion (below the horizontal dashed line) while the 7 samples above the dashed line failed the QC criterion.

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