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Table 1 Demographic of the Study Population and Biologic Samples1

From: Quality control in microarray assessment of gene expression in human airway epithelium

Parameter

Healthy nonsmoker

Healthy smoker

Symptomatic smoker

Lone emphysema with normal spirometry6

COPD7

 

Trachea

Large airway

Small airway

Trachea

Large airway

Small airway

Trachea

Large airway

Small airway

Small airway

Small airway

n

17

21

35

15

32

44

3

4

10

22

20

Age

42 ± 7

42 ± 9

43 ± 10

43 ± 7

44 ± 6

44 ± 6

36 ± 6

39 ± 6

41 ± 10

49 ± 7

52 ± 8

Gender2

13/4

15/6

26/9

13/2

22/10

31/13

2/1

3/1

5/5

16/6

16/4

Ancestry3

6/4/0/7

8/3/1/9

15/4/1/15

5/5/0/5

7/4/0/21

11/4/0/29

0/2/0/1

1/2/0/1

3/3/0/4

4/1/0/17

8/4/1/7

Smoking history

<1.0

<1.0

<1.0

28 ± 16

28 ± 18

28 ± 16

14 ± 4

16 ± 9

21 ± 13

31 ± 18

38 ± 23

Pulmonary function parameters4

   FVC

111 ± 16

105 ± 11

109 ± 11

108 ± 11

109 ± 12

109 ± 12

116 ± 6

113 ± 7

110 ± 109

102 ± 11

93 ± 23

   FEV1

111 ± 18

101 ± 26

105 ± 21

108 ± 13

109 ± 13

109 ± 14

112 ± 16

112 ± 13

108 ± 20

97 ± 12

72 ± 22

   FEV1/FVC

83 ± 7

82 ± 6

80 ± 7

82 ± 6

81 ± 5

81 ± 5

80 ± 6

81 ± 3

81 ± 13

79 ± 4

61 ± 9

   TLC

106 ± 17

99 ± 14

104 ± 13

100 ± 8

102 ± 12

100 ± 12

106 ± 4

108 ± 4

104 ± 19

93 ± 13

105 ± 22

   DLCO

110 ± 9

101 ± 18

101 ± 17

94 ± 7

96 ± 11

96 ± 11

92 ± 14

95 ± 13

94 ± 18

65 ± 8

73 ± 19

Average # of cells recovered (×106)

5.4

6.8

5.7

4.4

6.4

6.4

6.6

7.6

6.2

5.9

6.3

Cell differential5

   % epithelial

100 ± 0.2

100 ± 0.7

100 ± 0.6

100 ± 0.2

100 ± 0.7

100 ± 0.5

100 ± 0.0

100 ± 0.6

100 ± 0.4

99 ± 0.8

99 ± 1.7

   % inflammatory

0.1 ± 0.2

0.3 ± 0.7

0.3 ± 0.6

0.1 ± 0.2

0.3 ± 0.7

0.2 ± 0.5

0.0

0.4 ± 0.6

0.4 ± 0.4

0.6 ± 0.8

1.5 ± 1.7

   % ciliated

49 ± 7.1

55 ± 3.9

77 ± 5.6

27 ± 8.2

49 ± 9.0

72 ± 6.7

26 ± 2.8

47 ± 16

76 ± 5.4

73 ± 8.7

69 ± 2.8

   % secretory

6.6 ± 4.0

12 ± 4.0

6.8 ± 3.5

8.8 ± 4.4

11 ± 4.1

7.1 ± 3.0

12 ± 4.6

14 ± 1.2

5.9 ± 3.0

9.7 ± 7.2

12 ± 2.9

   % basal

29 ± 8.6

20 ± 3.4

9.1 ± 3.4

39 ± 5.2

24 ± 5.6

9.9 ± 3.3

37 ± 5.4

17 ± 10

10 ± 2.5

9.9 ± 4.8

8.2 ± 2.3

   % undifferentiated

15 ± 6.0

13 ± 3.8

7.3 ± 3.6

25 ± 11

15 ± 7.7

11 ± 5.6

25 ± 1.0

22 ± 6.0

7.8 ± 1.1

7.3 ± 2.4

9.6 ± 1.8

  1. 1 Data is presented as mean ± standard deviation.
  2. 2 Male/Female.
  3. 3 European/Hispanic/Asian/African.
  4. 4 Pulmonary function testing parameters are given as percent of predicted value with the exception of FEV1/FVC, which is reported as % observed; FVC - forced vital capacity, FEV1 - forced expiratory volume in 1 sec, TLC - total lung capacity, DLCO - diffusing capacity. For individuals with COPD, FVC, FEV1, and FEV1/FVC are post-bronchodilator values.
  5. 5 % epithelial and inflammatory are based on the total number of cells recovered; % ciliated, secretory, basal and undifferentiated cells are based on the total number of epithelial cells recovered.
  6. 6 Lone emphysema with normal spirometry smokers.
  7. 7 COPD smoker, GOLD stage I n = 9, II n = 9, III n = 2.
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BMC Genomics

ISSN: 1471-2164

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