Skip to main content

Table 1 Demographic of the Study Population and Biologic Samples1

From: Quality control in microarray assessment of gene expression in human airway epithelium

Parameter Healthy nonsmoker Healthy smoker Symptomatic smoker Lone emphysema with normal spirometry6 COPD7
  Trachea Large airway Small airway Trachea Large airway Small airway Trachea Large airway Small airway Small airway Small airway
n 17 21 35 15 32 44 3 4 10 22 20
Age 42 ± 7 42 ± 9 43 ± 10 43 ± 7 44 ± 6 44 ± 6 36 ± 6 39 ± 6 41 ± 10 49 ± 7 52 ± 8
Gender2 13/4 15/6 26/9 13/2 22/10 31/13 2/1 3/1 5/5 16/6 16/4
Ancestry3 6/4/0/7 8/3/1/9 15/4/1/15 5/5/0/5 7/4/0/21 11/4/0/29 0/2/0/1 1/2/0/1 3/3/0/4 4/1/0/17 8/4/1/7
Smoking history <1.0 <1.0 <1.0 28 ± 16 28 ± 18 28 ± 16 14 ± 4 16 ± 9 21 ± 13 31 ± 18 38 ± 23
Pulmonary function parameters4
   FVC 111 ± 16 105 ± 11 109 ± 11 108 ± 11 109 ± 12 109 ± 12 116 ± 6 113 ± 7 110 ± 109 102 ± 11 93 ± 23
   FEV1 111 ± 18 101 ± 26 105 ± 21 108 ± 13 109 ± 13 109 ± 14 112 ± 16 112 ± 13 108 ± 20 97 ± 12 72 ± 22
   FEV1/FVC 83 ± 7 82 ± 6 80 ± 7 82 ± 6 81 ± 5 81 ± 5 80 ± 6 81 ± 3 81 ± 13 79 ± 4 61 ± 9
   TLC 106 ± 17 99 ± 14 104 ± 13 100 ± 8 102 ± 12 100 ± 12 106 ± 4 108 ± 4 104 ± 19 93 ± 13 105 ± 22
   DLCO 110 ± 9 101 ± 18 101 ± 17 94 ± 7 96 ± 11 96 ± 11 92 ± 14 95 ± 13 94 ± 18 65 ± 8 73 ± 19
Average # of cells recovered (×106) 5.4 6.8 5.7 4.4 6.4 6.4 6.6 7.6 6.2 5.9 6.3
Cell differential5
   % epithelial 100 ± 0.2 100 ± 0.7 100 ± 0.6 100 ± 0.2 100 ± 0.7 100 ± 0.5 100 ± 0.0 100 ± 0.6 100 ± 0.4 99 ± 0.8 99 ± 1.7
   % inflammatory 0.1 ± 0.2 0.3 ± 0.7 0.3 ± 0.6 0.1 ± 0.2 0.3 ± 0.7 0.2 ± 0.5 0.0 0.4 ± 0.6 0.4 ± 0.4 0.6 ± 0.8 1.5 ± 1.7
   % ciliated 49 ± 7.1 55 ± 3.9 77 ± 5.6 27 ± 8.2 49 ± 9.0 72 ± 6.7 26 ± 2.8 47 ± 16 76 ± 5.4 73 ± 8.7 69 ± 2.8
   % secretory 6.6 ± 4.0 12 ± 4.0 6.8 ± 3.5 8.8 ± 4.4 11 ± 4.1 7.1 ± 3.0 12 ± 4.6 14 ± 1.2 5.9 ± 3.0 9.7 ± 7.2 12 ± 2.9
   % basal 29 ± 8.6 20 ± 3.4 9.1 ± 3.4 39 ± 5.2 24 ± 5.6 9.9 ± 3.3 37 ± 5.4 17 ± 10 10 ± 2.5 9.9 ± 4.8 8.2 ± 2.3
   % undifferentiated 15 ± 6.0 13 ± 3.8 7.3 ± 3.6 25 ± 11 15 ± 7.7 11 ± 5.6 25 ± 1.0 22 ± 6.0 7.8 ± 1.1 7.3 ± 2.4 9.6 ± 1.8
  1. 1 Data is presented as mean ± standard deviation.
  2. 2 Male/Female.
  3. 3 European/Hispanic/Asian/African.
  4. 4 Pulmonary function testing parameters are given as percent of predicted value with the exception of FEV1/FVC, which is reported as % observed; FVC - forced vital capacity, FEV1 - forced expiratory volume in 1 sec, TLC - total lung capacity, DLCO - diffusing capacity. For individuals with COPD, FVC, FEV1, and FEV1/FVC are post-bronchodilator values.
  5. 5 % epithelial and inflammatory are based on the total number of cells recovered; % ciliated, secretory, basal and undifferentiated cells are based on the total number of epithelial cells recovered.
  6. 6 Lone emphysema with normal spirometry smokers.
  7. 7 COPD smoker, GOLD stage I n = 9, II n = 9, III n = 2.