Figure 1

Comparison of detoxification enzymes activities between the insecticide-resistant strain Vauclin and the susceptible strain Bora-Bora. A) P450 activities were measured with the ECOD method [63] on 20 μg microsomal proteins after 15 min and expressed as pmol of 7-OH produced/mg microsomal protein/minute (± SE). B) GST activities were measured with the CDNB method [64] on 200 μg cytosolic proteins during 1 min and expressed as nmol of conjugated CDNB/μg protein/min (± SE). α-esterase (C) and β-esterase (D) activities were measured with the naphthyl acetate method [65] on 30 μg cytosolic proteins after 15 min and expressed as μmol α- or β-naphthol produced/mg protein/minute (± SE). For each strain and each life stage, 3 independent biological replicates were analyzed and measures were repeated 15, 15 and 30 times for P450, GST and esterase activities respectively. Statistical comparison of enzyme activities between the Vauclin and Bora-Bora strains were performed at each life stage separately with a Mann and Whitney's test (* p < 0.05, ** p < 0.01, *** p < 0.001).