Figure 1

Fragment sizing with ABI 3730xl and frequency distribution of fragment sizes using GS1200Liz size standard. Figure 1A shows an example of fingerprinting profile of a digested BAC clone using GS1200Liz as a size standard. The fingerprinting of each BAC involved digestion with five restricted enzymes and labeling with four fluorescent dyes as described previously [22]. The size for each fragment was calculated based on co-migration of size standard in the capillary. Figure 1B shows the frequency of fragments with different sizes in 14,231 fingerprinted Brachypodium BAC clones. Large peaks represent vector fragments that appear in high frequencies. The red line defines the threshold for high frequency fragments derived from BAC inserts. Fragments with a frequency above the threshold were removed prior to contig assembly due to their likely origin from repetitive sequences.