Figure 4

High-resolution analysis of CREB binding at uni-directional and bi-directional promoters. Depicted are CREB binding data from two representative promoter regions, the length (bp) of each is indicated above the graph. The log2 ratio for each probe spanning the promoter region was determined for the CREB ChIP-chip (plotted in red) or HaloCHIP-chip (plotted in blue) data. Positions of transcription start sites (TSS) are shown with arrows indicating the direction of transcription with length of exons in blocks and introns drawn as lines. Positions of full and half CRE sites relative to the location within the promoter region are indicated below by blue dots. A. Example of CREB binding to a unidirectional promoter, ALS2, where peak binding is observed upstream of the TSS and localized to the CRE consensus sites. B. Example of CREB binding to a bidirectional promoter, METTL4 and NDC80, where peak enrichment is located downstream of the TSSs, but the peak enrichment does not localize to CRE consensus sites.