A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays

BMC Genomics

Table 1 CREB HaloCHIP-chip positive and negative predictive values.

Twelve promoters each from the Top 1%, Top 5%, and Top 10% categories of the HaloCHIP-chip data were chosen to determine the positive predictive value and 36 promoters were chosen from the Bottom 50% of the list for determination of the negative predictive value. SYBR green qPCR was used to determine the presence of these promoters in triplicates samples of HT-CREB experimental and untransfected control HaloCHIP DNA and promoters were considered enriched if having a signal:background ratio ≥ 2-fold between the experimental and control sample. Based upon these results, further analysis of the HaloCHIP-chip data was limited to promoters identified within the Top 5% of the list.

ISSN: 1471-2164