Figure 2

Formation of the TIGD1 gene in primates. (A) Schematic representation pre- and post- insertion of the TIGD1 ancestral TE with locations of amplification primers (red arrows) used to screen for the presence of the TIGD1 gene in mammalian species and CpG island (green bars). Non-coding exons are denoted by narrow boxes and coding exons by larger shaded boxes with the direction of transcription indicated by arrowheads. (B) Molecular phylogenetic analysis of the domestication of TIGD1 in primates and a summary of TIGD1 bioinformatic or PCR screening assay. (+) denotes the presence of the TIGD1 gene and (-) denotes the absence of this gene within specific species. Phylogenetic tree of primate lineage displayed.