Figure 5

Transcriptional activity and CpG island genomic environment, pre and post insertion of TIGD1. (A) Promoter region of the mouse Eif4e2 gene shows a predominantly unidirectional grouping of cap analysis gene expression (CAGE) tag transcription start sites in the plus direction (brown vertical bars) together with some detectable activity in the minus direction (blue vertical bars). (B) After the insertion of the TIGD1 element in the primate lineage, the human TIGD1-EIF4E2 promoter region displays a lengthening of the CpG island and bidirectional transcription of the TIGD1 and EIF4E2 genes, blue and brown vertical bars, respectively. The Riken CAGE transcription start sites are overlayed on a 1,500 bp window for mouse and human genome assemblies mm5 and hg17, respectively.