Figure 8
Genes involved in tissue protection, clearance and reparation. A. Real-time qPCR versus microarray analyses. Data for qPCR are ΔΔCt ± SE of 10 HR and 10 LR versus 8 fish in the unchallenged control and data for microarray are mean log-ER ± SE of 6 HR fish hybridized to a pooled sample of 10 LR fish. Differences between HR and LR determined with microarrays and qPCR are shown. Different capital letters (A, B) denote a difference between challenged fish (HR and LR) while small letters (a, b) denote a difference between challenged and control fish (ANOVA, P < 0.05). FN1 - fibronectin, ABCB4 - liver bile salt export pump. B. Microarray results, examples of differentially expressed genes with significant differences between vaccinated and unvaccinated fish (t-test, n = 6 and 5, p < 0.05). Results for unvaccinated fish are not shown, data are log2-ER ± SE.