Analysis of organs derived from control and scNT-MUC piglets. A) Microscopic analysis revealed evidence of severe calcification in scNT-MUC-derived placenta (e) and kidney (h). Lung (f) and liver (g) sections derived from scNT clones with MUC showed abnormal stromal cell proliferation, peliosis in the sinusoidal space and tubular degeneration. B) De-waxed paraffin sections of placenta, lung, liver and kidney from control (n = 3) and scNT-MUC (n = 6) piglets were subjected to a TUNEL assay. Increased apoptosis was evident in several organs (e-h) derived from scNT clones with MUC, while few TUNEL-positive cells were observed in control organs (a-d). Apoptosis is indicated by brown or green coloration. CT, cytotrophoblast; ST, syncytotrophoblast; A, alveoli; As, alveolar sac; B, blood vessels; C, calcification; Fv, floating villi; H, hepatocytes; IS, interalveolar septum; Bbl, border between lobules; Rb, respiratory bronchiole; PT, proximal tubules, S, stromal cell. Magnification, 200×.