| Site numbers | Genes | % all 3'-end exon sites |
---|---|---|---|
i. Single-block ESTs | Â | Â | Â |
   No. of poly(A/T)-tailed ESTs | 494,529 |  |  |
   No. of non-poly(A/T)-tailed ESTs | 2,829,147 |  |  |
   No. of final 3'-end exon sites | 10,844 | 5,670 | 100% |
   No. supported by non-poly(A/T)-tailed ESTs | 7,676 | 4,599 | 71% |
ii. Validation tests | Â | Â | Â |
   Blast hit against all known cDNAs | 2,957 | 2,257 | 27% |
   Support from EST clone IDs | 1,155 | 1,001 | 11% |
   Support from Affymetrix transcripts | 5,475 | 3,627 | 50% |
   RT-PCR experiments | 10/26 |  |  |
iii. Overlapping with other AS and APA databases | Â | Â | Â |
   PolyA_DB2 | 1,410 | 1,235 | 13% |
   ASAP II database | 613 | 554 | 6% |
   ASTD database | 1,250 | 1,115 | 11% |
   Muro et al's data | 4,046 | 2,895 | 37% |
iv. PAS analysis | Â | Â | Â |
   PAS positive | 7,051 | 4,292 | 65% |
v. Map domains | Â | Â | Â |
   At least one domain deletion | 7,641 | 4,142 | 70% |
   Transmembrane helix deletion | 1,616 | 945 | 15% |