Table 1 Summary of novel human intronic 3'-end exon sites

i. Single-block ESTs

   No. of poly(A/T)-tailed ESTs

494,529

   No. of non-poly(A/T)-tailed ESTs

2,829,147

   No. of final 3'-end exon sites

10,844

5,670

100%

   No. supported by non-poly(A/T)-tailed ESTs

7,676

4,599

71%

ii. Validation tests

   Blast hit against all known cDNAs

2,957

2,257

27%

   Support from EST clone IDs

1,155

1,001

11%

   Support from Affymetrix transcripts

5,475

3,627

50%

   RT-PCR experiments

10/26

iii. Overlapping with other AS and APA databases

   PolyA_DB2

1,410

1,235

13%

   ASAP II database

613

554

6%

   ASTD database

1,250

1,115

11%

   Muro et al's data

4,046

2,895

37%

iv. PAS analysis

   PAS positive

7,051

4,292

65%

v. Map domains

   At least one domain deletion

7,641

4,142

70%

   Transmembrane helix deletion

1,616

945

15%