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Figure 1 | BMC Genomics

Figure 1

From: A comprehensive resource of drought- and salinity- responsive ESTs for gene discovery and marker development in chickpea (Cicer arietinum L.)

Figure 1

Summary of ESTs generated from drought- and salinity-responsive chickpea genotypes. The figure shows a flowchart of generation and analysis of ESTs in four groups. ESTs generated and analysed for drought-responsive tissues have been shown in A, for salinity-responsive tissues in B, all ESTs generated in this study in C, and all chickpea ESTs analysed in D. A: Four different drought stress treatments were imposed on each of chickpea genotypes ICC 4958 and ICC 1882. Raw sequences (RS) were trimmed to generate high quality ESTs (HQS). Cluster analysis of 10,996 sequences provided 4,558 unigenes (UG-I), B: ESTs were generated from salinity challenged root tissues of JG 11 and ICCV 2. Sequence trimming provided 7,439 high quality sequences. Clustering analysis of these sequences yielded 2,595 unigenes (UG-II), C: ESTs generated from four genotypes as shown in A and B were analysed together that provided a set of 6,404 unigenes (UG-III), D: ESTs generated in this study were analysed together with 7,097 public domain ESTs. Clustering and assembly analysis resulted in 9,569 unigenes (UG-IV) for chickpea.

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