Figure 5
Clustered correlation map of differentially expressed genes under stress. Hierarchial clustering of ESTs representing genes involved in drought- and salinity- stress responses was done using HCE version 2.0 beta web tool. The dendrogram on top illustrates the relationship among 10 cDNA libraries: 1. ICC 4958_PEG_Induction, 2. ICC 4958_Dehydration, 3. ICC 4958_Drought_Glasshouse, 4. ICC 4958_Drought_Field, 5. ICC 1882_PEG_Induction, 6. ICC 1882_ PEG, 7. ICC 1882_Drought_Glasshouse, 8. ICC 1882_Drought_Field, 9. JG 11_Salinity, 10. ICCV 2_Salinity. Clustering of highly expressed ESTs (normalized using R statistics, R>8) into four major clusters (indicated by vertical colour bars) were further subclustered into nine groups based on library specificity (I, IIa, IIb, IIc, IIIa, IIIb, IIIc, IIId and IV). A colour map/heat map with red representing normalized expression values greater than the mean, green colour representing expression less than the mean and colour intensities in between representing the magnitude of the deviation from the mean can be observed. Colour scale (from green to red) represents the range of expression level.