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Table 4 Relative gene expression of the non-reference genes in D. magna after normalisation in the Chaoborus kairomone experiment.

From: Target gene approaches: Gene expression in Daphnia magna exposed to predator-borne kairomones or to microcystin-producing and microcystin-free Microcystis aeruginosa

 

Relative expressiona

Gene

Calibrator

Invertebrate predator

SDb

18S

1.0000 ± 0.0033

1.8183 ± 0.0069

0.5786

28S

1.0000 ± 0.0065

1.2141 ± 0.0013

0.1514

cyclophilin

1.0000 ± 0.0012

0.3877 ± 0.0022

0.4329

UBC

1.0000 ± 0.0042

1.7394 ± 0.0042

0.5229

  1. D. magna was raised in either a predator-free environment ('Calibrator'), or in incubation water of larvae of Chaoborus flavicans ('Invertebrate predator').
  2. aValues are mean of n = 3 replicates ± SD. Expression levels are displayed relative to the mean control level
  3. bSD is the variation of one reference gene across treatments
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BMC Genomics

ISSN: 1471-2164

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