Figure 3

Analysis of XBP1 splicing and DDIT3/CHOP subcellular localization in SH-SY5Y cells treated with UCB. (A) mRNA levels of the spliced form of XBP1 (s - spliced, u - unspliced), indicator of ER stress, were examined by RT-PCR after 24 h of treatment. The amplified products were separated on a 2% agarose gel and visualized with ethidium bromide staining. Data are representative of n ≥ 2 separate experiments per condition point. (B) The expression of CHOP (shown in red) was examined with immunocytochemistry as described in Methods. Representative images are shown for SH-SY5Y cells not treated (untreated) or treated with vehicle (DMSO 0.6%) or UCB (Bf 140 nM). The nuclei (shown in blue) were visualized with DAPI staining. Bar = 10 μm.