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Figure 1 | BMC Genomics

Figure 1

From: Ligand-specific sequential regulation of transcription factors for differentiation of MCF-7 cells

Figure 1

Time-dependent gene expression of EGF- or HRG-induced transcription factors. (A) Gene expression of 2,352 human transcription factors in EGF- and HRG-treated MCF-7 cells was measured by qRT-PCR. The index value I for these transcription factors was calculated and its distribution is shown. The fitted normal distribution is shown as a dotted curve. Red bars represent genes whose I value is greater than 3.0, with five transcription factors (c-FOS, EGR4, FOSL-1, FHL2 and DIPA) satisfying this criterion. (B-D) qRT-PCR and GeneChip gene expression analyses were performed using growth factor-treated MCF-7 cells. The expression of mRNA after treatment of the cells with 10 nM HRG or EGF was monitored by qRT-PCR for up to 6 hrs and for up to 72 hrs using a GeneChip microarray system. The expression profiles of five transcription factors with high I value are shown. (B) Gene expression time-course of five transcription factors measured by qRT-PCR. (C) Gene expression time-course of five transcription factors measured by GeneChip. (D) HRG-induced gene expression measured using a GeneChip microarray system. These five transcription factors show sequential peaks of mRNA expression.

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