TY - JOUR AU - Steuernagel, Burkhard AU - Taudien, Stefan AU - Gundlach, Heidrun AU - Seidel, Michael AU - Ariyadasa, Ruvini AU - Schulte, Daniela AU - Petzold, Andreas AU - Felder, Marius AU - Graner, Andreas AU - Scholz, Uwe AU - Mayer, Klaus FX AU - Platzer, Matthias AU - Stein, Nils PY - 2009 DA - 2009/11/20 TI - De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barley JO - BMC Genomics SP - 547 VL - 10 IS - 1 AB - De novo sequencing the entire genome of a large complex plant genome like the one of barley (Hordeum vulgare L.) is a major challenge both in terms of experimental feasibility and costs. The emergence and breathtaking progress of next generation sequencing technologies has put this goal into focus and a clone based strategy combined with the 454/Roche technology is conceivable. SN - 1471-2164 UR - https://doi.org/10.1186/1471-2164-10-547 DO - 10.1186/1471-2164-10-547 ID - Steuernagel2009 ER -