De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barley

Table 1 Summary of 454 sequencing for 96 BACs in two sets.

set	BAC	num reads	vec	coli	num reads w/o vec/coli	avgread length (bp)	min cov	max cov	av cov
1	48	505,448	3%	6%	460,168	225	12.5×	37.3×	21.6×
2	48	715,902	3%	10%	626,155	217	9.4×	64.4×	26.6×
both	96	1,221,350	3%	8%	1,086,323	221	9.4×	64.4×	24.3×

ISSN: 1471-2164