De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barley

Table 2 Summary of best MIRA assembly results for 96 BACs in two sets and four reference BACs included in both sets.

set	BAC	largest contig bp	sum of contigs >1 kb bp	N50 for contigs >1 kb bp	N80 for contigs >1 kb bp	N90 for contigs >1 kb bp	avg cov	0.5-10 kb	10-50 kb	50-100 kb	>100 kb
1	all (48)	66,682	4,972,546	59,359	34,037	21,204	22×	488	61	34	4
2	all (48)	58,210	5,134,266	38,959	27,816	20,140	27×	546	97	24	3
both	all (96)	62,466	10,106,812	48,945	30,926	20,671	24×	1.034	158	58	7
1	184G09	120,316	120,316	120,316	120,316	120,316	18×	-	-	-	1
1	259I16	74,161	125,700	74,161	11,492	8,588	19×	4	2	1	-
1	631P08	95,649	100,966	95,649	95,649	95,649	22×	2	-	1	-
1	711N16	75,684	101,682	75,684	12,600	11,564	24×	-	2	1	-
2	184G09	91,195	121,589	91,195	28,663	28,663	25×	1	1	1	-
2	259I16	44,395	131,950	10,249	7,210	5246	14×	9	4	-	-
2	631P08	52,664	102,141	26,440	18,577	18,577	24×	3	2	1	-
2	711N16	28,312	115,582	15,998	15,998	11,527	31×	4	5	-	-

ISSN: 1471-2164