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Table 3 GUS expression in the F2 generation of gene trap lines

From: An Ac/Ds-mediated gene trap system for functional genomics in barley

GTDs B line Gene trap line No. plants analysed No. plants with GUS Organ with GUS activity (no. explants)
2a/d GT32 6 2 grain (1), seedling (1)
  GT73 4 1 grain (1)
23 GT33 4 - -
  GT29 4(1c) 2 pollinated floret (1), seedling (1)
  GT39 1 - -
  GT41 5 3 pollinated floret (13), grain (4), seedling (1)
31B GT49 4 2 pollinated floret (2), seedling (1)
  GT54 5 5 immature (7)/pollinated (1) floret, grain (10), seedling (15)
6a/c GT70 4 1 pollinated floret (1), node (1)
  GT67 6 4 node (4), leaf (1)
  GT4 7(1a) - -
31A GT38 6 1 seedling (1)
  GT52 5(1d/1e) 4 node (1), grain (10)
16 GT19 4b 1 grain (1)
  GT37 8 2 grain (4), seedling (1)
  GT40 4 - -
14A GT13 4(2b/2c) 2 grain (1), seedling (7)
  GT22 5(4b/1c) 4 grain (2), seedling (17)
11 GT82 3 3 immature floret (1), node (3), leaf (1)
  GT66 3(f) 2 pollinated floret (3), grain (1)
  GT80 4 4 immature floret (3), pollinated floret (2), node (4), leaf (1), grain (9), seedling (2)
10 GT9 7(7b) 2 grain (2), seedling (2)
  GT2 7(6b/1c) 5 grain (2), seedling (4)
  GT6 7(5b/2c) 4 grain (6), seedling (3)
14B GT23 4(2a/1d) 1 seedling (1)
  GT15 8(1b/3c) 7 pollinated floret (9), grain (12), seedling (17)
26 GT35 4 4 immature floret (41), pollinated floret (32)
  GT63 8 7 immature floret (3), grain (23), seedling (2)
Total   141 72  
  1. (No. plants a)Only seedlings were analysed
  2. (No. plants b)Only grains and seedlings were analysed
  3. (No. plants c)Only pollinated florets, grains and seedlings were analysed
  4. (No. plants d)No pollinated florets were analysed
  5. (No. plants e)No grains were analysed
  6. (No. plants f)Only immature florets, nodes and leafs were analysed