Genome reannotation of Escherichia coli CFT073 with new insights into virulence

BMC Genomics

Table 1 Overview of the differences between the original RefSeq annotation and the reannotation

	Original annotation (RefSeq: NC_004431)	Reannotation
Genome length	5,231,426 bp
plasmids	None
G+C%	50.47%
Protein-coding genes	5,339	5,030
tRNAs	89
rRNAs	21
Miscellaneous RNAs^a	6	46
Backbone genes^b	4,550 (4,440 protein-coding genes, 85 tRNA genes, 21 rRNA genes, and 4 miscellaneous RNA genes)	4,328 (4,178 protein-coding genes, 85 tRNA genes, 21 rRNA genes, and 44 miscellaneous RNA genes)
Genomic island genes^c	905 (899 protein-coding genes, 4 tRNA genes, and 2 miscellaneous RNA genes)	851 (845 protein-coding genes, 4 tRNA genes, and 2 miscellaneous RNA genes)
Cryptic prophages	5

^aThe concept of miscellaneous RNA here includes tRNAs, rRNAs and all other RNAs;
^bIn this comparison, we define the genes other than those in the genomic island regions as backbone genes;
^cThe genes located in genomic island regions (data conducted by Lloyd and et al [47]).

ISSN: 1471-2164