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Table 2 Oligonucleotide probes design.

From: Combining next-generation pyrosequencing with microarray for large scale expression analysis in non-model species

  NN N TCs
Unigenes 34,477 29,627 33,638
Designed oligonucleotide probes 17,843 29,393 32,771
Probes specific for 1 sequence 16,990 28,187 25,658
Probes recognizing 2-3 sequences 801 1,177 6,326
Probes recognizing 4-5 sequences 52 29 787
Oligonucleotide probes mapping to genome 16,500 25,879 26,578
Probes mapping to a unique position 14,146 21,869 21,831
Probes mapping to a 2-3 positions 1,594 2,654 2,848
Probes mapping to 4-5 positions 301 506 566
Probes mapping to more than 5 positions 459 850 1,333
Probes not mapping to genome 1,343 3,514 6,193
  1. Oligonucleotide probes design was performed on the unigenes identified in the non-normalized (NN) and normalized (N) libraries and on the TCs comprised in VvGI.