Figure 2

Isolation and transcriptomic analysis of microvesicular RNA. A) Bioanalyzer results of total RNA isolated from microvesicles and SW480 cells. The third and fourth peaks correspond to 18S and 28S rRNA, respectively. Images of gel electrophoresis are also shown. B) Gel electrophoresis of microvesicular and cellular β-actin and β-catenin mRNA amplified by RT-PCR. C) Scatterplots of microvesicular and cellular mRNA expression levels showing log2 intensities in the first and the second arrays (Pearson's correlation coefficients: ρ = 0.9917 and 0.9920 for microvesicular RNA and cellular RNA, respectively). D) Scatterplot of median log2 intensities of the four microvesicle replicates versus those of their donor cells (ρ = 0.9778).