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Figure 5 | BMC Genomics

Figure 5

From: Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells

Figure 5

A biological network describing the cellular processes overrepresented by microvesicle-enriched mRNAs. A) A heat map illustrating the expression patterns of the 1,702 genes that exhibited a more than 2-fold difference in the mRNA level between microvesicles and cells. For clarity, the expression of each is scaled to a mean of zero with unit variance across all eight replicates. The colors represent the relative increase (red) or decrease (green) in the expression level of the corresponding gene in microvesicles compared with the level in cells: 241 and 1,461 genes were overexpressed in microvesicles and in cells, respectively. B) A GO subtree showing cell cycle-related GOBPs overrepresented by the 241 microvesicle-enriched genes. The color scheme of Figure 4 is repeated. Notably, cell cycle-related GOBPs were strongly overrepresented by 27 mRNAs, as indicated by the dark-red node colors (see text for details). C) A hypothetical network reconstructed using the 27 microvesicle-enriched mRNAs belonging to the cell cycle-related GOBPs in (B) and their first and second interaction neighbors obtained from protein-protein interaction databases (see Materials and methods). The yellow nodes represent the 27 cell cycle-related microvesicle-enriched genes: 20 of the 27 genes closely interact with cell cycle-related processes. The lines between the nodes represent protein-protein interactions. The network shows that the microvesicle-enriched genes closely interact with key pathways in the cell cycle. D) Cluster heat map comprising 36 of the 241 microvesicle-enriched mRNAs showing consistent differential expression patterns across patients in two independent CRC datasets (GSE2109 and GSE5206). The diamond nodes in the network (C) represent the 17 nodes shared by the 27 cell cycle-associated mRNAs and 36 mRNAs; of the 17 nodes, 15 (labeled in red) are associated with M-phase-related processes.

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