Skip to main content
Figure 3 | BMC Genomics

Figure 3

From: Development and bin mapping of a Rosaceae Conserved Ortholog Set (COS) of markers

Figure 3

Development of primers in adjacent exons that flank the same intron. The output of the python contig software tool [40] allows the determination of the intron position based on the Arabidopsis genome sequence and EST constitution of RosCOS. The Rosaceae consensus sequence (red), composed of Fragaria (grey), Malus (white) and Prunus (blue) ESTs, and was compared to the Arabidopsis genome (green). The intron (Δ) and flanking positions (yellow blocks) were used to develop universal primers using Primer3 v. 0.4.0 [41].

Back to article page
\